A Semiautomated Enzymatic Method for Urinary Glucose
نویسندگان
چکیده
منابع مشابه
Semiautomated assay of normal concentrations of urinary glucose by an enzymatic fluorometric technic.
A semiautomated, enzymatic, fluorometric method for the determination of the low glucose concentrations normally present in urine from subjects in the fasting state is described. The method is developed from a manual, enzymatic, fluorometric technic for the determination of urinary glucose using hexokinase (HK) and glucose-6phosphate dehydrogenase (G-6-PDH). The procedure is based on measuremen...
متن کاملAn enzymatic method for glucose determination in body fluids.
ENZYMATIC REACTIONS, BECAUSE of their great specificity, are among the most valuable analytic tools at the disposal of the chemist. The method for glucose here described represents an adaptation of such biologic reagents and is a distinct departure from sugar methods now in common use. It is based upon a unique principle communicated to us by Keston (1) prior to publication (2). Traditional met...
متن کاملAn Enzymatic Method for Glucose Quantitation in Normal Urine.
1 (JRING INVESTIGATIONS of the renal reabsorptive mechanism for glucose, a method to quantitate adequately the glucose of normal urine (1-3) was required. The nonspecificity of classic methods for reducing sugars makes them inadequate for application to normal urine. Specific glucose oxidase methods have not yielded adequate recoveries when applied to normal, dilute urine. I have adopted the en...
متن کاملSemiautomated method for quantitative urine culture.
Kemp RB. Effect of the removal of cell surface sialic acid on aggregation in vitro. Nature 1968;218: 1255. 8Steinhardt RA, Lundin L, Mazia D. Bioelectric responses of the echinoderm egg to fertilisation. Proc Natl Acad Sci 1971;68:2426-30. 9 Macieira-Coelho A, Avrameas S. Modulation of cell behaviour in vitro by the substratum in fibroblastic and leukaemic mouse cell lines. Proc Natl Acad Sci 1...
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An accurate, reproducible method for determining the infarct volumes of gray matter structures is presented for use with presently available image analysis systems. Areas of stained sections with optical densities above that of a threshold value are automatically recognized and measured. This eliminates the potential error and bias inherent in manually delineating infarcted regions. Moreover, t...
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ژورنال
عنوان ژورنال: Public Health Reports (1896-1970)
سال: 1966
ISSN: 0094-6214
DOI: 10.2307/4592821